Effects of vancomycin and ramoplanin on gene expression in Clostridioides difficile

We compared transcriptomes of wild-type and ?vanS strains of Clostridioides difficile 630 growing in the presence or absence of peptidoglycan-targeting antibiotics, vancomycin or ramoplanin. VanS is a histidine kinase of a two-component system that regulates expression of the vancomycin-induced vanG operon. Overall design: Comparative RNAtag-Seq of wild-type and vanS null mutant cells in the presence or absence of vancomycin or ramoplanin