Transcriptome analysis of human memory T cell subsets from blood before and after ex-vivo CD3/CD28 stimulation
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https://www.ncbi.nlm.nih.gov/sra/SRP342853
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Naive, central memory (TCM), effector memory (TEM), and terminally differentiated effector memory RA (TEMRA, CD8+ only) T cell subsets were FACS separated from PBMC samples of four human donors using CCR7 and CD45RA as distinguishing cell surface markers. Samples were split and either immediately isolated, or incubated for 42-48 hours with anti-CD3/CD28 beads for ex-vivo stimulation. Overall design: RNA-seq on naive and memory subsets isolated from human blood samples
创建时间:
2023-05-03



