Identification of a novel antitumor CD4+ T cell cluster orchestrating antitumor immunity

scRNAseq-based unsupervised clustering and clonotyping revealed that Th1/17 and CCR6 SP clusters formed metacluster Th7R, which was distinct from Th1 or Th17, characterized by high expression of the IL-7 receptor. Use of this cluster to assess antitumor CD4+ T cell immunity from the peripheral blood and to predict the efficacy of immune checkpoint inhibitors will pave the way for novel antitumor immunotherapy strategies for patients. Overall design: Investigating how differences in gene expression and TCR repertoires of T cells in blood affect the response of pembrolizumab therapy. All samples in this dataset were obtained from patients before receiving pembrolizumab therapy. GEX and TCR samples with the same P* identifier were obtained from the same set of cells.