Analysis of TCR repertoire in beta5t-deficient mice. Mus musculus

Proteasomes play an important role to produce peptides presented on MHC class I. Thymoproteasome which bears beta5t as a component of its catalytic subunit, is specifically expressed in cortical thymic epithelial cells and is thought crucial for the repertoire of self-peptide ligands yielding positive selection of CD8+ T cells. In this study, we aimed to understand how the decifiency of beta5t affect TCR repertore of CD8 T cells. CD44loCD62LloCD8+ T cells were sorted from splenocytes from beta5t-deficient and control mice. cDNA samples were prepared and subjected to RNAseq analysis on Illumina Genome Analyzer.