Trait data for the evolution of plasticity in pairwise competitive and mutualistic community of brewer's yeast

Phenotypic plasticity allows organisms to change their phenotypes in response to changing local conditions quickly in order to better function.  Plasticity in response to abitoic factors such as temperature, light, and salinity for example have been well documented.  What is less clear is how species interactions can influence the evolution of phenotypic plasticitiy as species adapt and interact with one another.  To study this question we used a synthetic mutualism based on genetically modified brewer's yeast (Saccharomyces cerevisiae) that overproduce a certain resource but cannot produce one needed by its partner strain.  In this study system, we paired one yeast strain that overproduces adenine, but cannot produce lysine, and another strain that overproduces lysine but cannot produce adenine. When placed together in media they can cross feed one another and form a mutualism.  We also paired strains that just had a knockout for adenine or lysine under competition.  The goal of the st..., Yeast strains were evolved for 4 weeks either alone, or with a competitor or mutualist strain.  Each strain was then phenotyped for growth rate and yield in low and high nutrient environments by assessing population via optical density.  Data were entered into MS Excel and used in R statistical software to evaluate the amount of change in traits and their plasticity., , # Trait data for the evolution of plasticity in pairwise competitive and mutualistic community of brewer's yeast

[https://doi.org/10.5061/dryad.5qfttdzgf](https://doi.org/10.5061/dryad.5qfttdzgf)

## Description of the data and file structure

Strains of genetically engineered brewer's yeast we evolved alone, with a competitor strain, or with a mutualist strain for four weeks.  Strains were then phenotyped for growth rate, yield and plasiticity in these traits.  

### Files and variables

#### File: YeastPP\_Data.xlsx

**Description:** Data used in statistical comparisons

##### Variables

* strain-- strain identification number
* adelyse-- does strain make adenine or lysine
* wo-- OP is overproduction and WT is wildtype production
* marker-- null alleles for histine or leucine to use in selective plating
* plateN-- which deepwell plate was strain evolved in
* location-- row and column information for well in deepwell plate
* genotype--which strains were growing in well
* treatment-- g...