Validation of Transcription Factors Highly Regulated by OpaR via qRT-PCR.

aFor the microarray data, the standard deviation of the normalized expression in four biological replicates of the ∆opaR1 strain was compared to three biological replicates of opaR+ strains.b RNA-Seq data is fold change of the opaR+ strain gene expression divided by the ∆opaR1 strain gene expression. Error for the ratios of normalized gene expression levels were conservatively estimated using the standard deviation ratios across the majority of genes with less than 4-fold change. The standard deviation for chromosome 1 is 1.53 and the error for chromosome 2 is 1.59.c The error in fold changes for each gene was estimated by computing the standard deviation for four replicates of the qRT-PCR measurements.Validation of Transcription Factors Highly Regulated by OpaR via qRT-PCR.