Histology and immunohistochemistry scoring of ear sections from VD and DD hapten treated ear biopsy samples (7 and 21 day sensitization periods).

Measures of pathology, including cellular infiltrate, vasculitis and thrombosis, and necrosis were scored from H&E preps on a 0–4 scale. In addition, CD3 immunostaining of ear biopsy samples was also performed and scored on a 0–4 scale. Depicted are the Lsmeans and standard errors of 4 ear punches per VD and DD treatment for both the 7 and 21 day sensitization studies and the p-value testing statistical significance of the difference between VD and DD groups within sensitization period.