Organic solvent stability study on MLipA in various hydrophobic and hydrophilic solvents.

Purfied lipase was incubated with 25% (v/v) organic solvent at a ratio of three volumes to one for durations of 1 h and 24 h at 35°C under 100 rpm. The activity was assayed after the incubation periods and relative activity was calculated against the control reaction (without addition of organic solvent) set as 100% (328.4 U/mg after 1 h incubation and 270.7 U/mg after 24 h incubation).