The effect of the pH on time of EOF (tEOF), the migration time (t), resolution (Rs) and separation selectivity (α) of folinic acid diastereomers.

Note: In pH range of 3.50–5.75, no peaks of folinic acid diastereomers were detected within 60 min. Each data is the mean of two injections.The enantioseparation parameters of CE were calculated as follows: Rs = 2(t2-t1) / (W2+W1); α = t2/t1; where t1 and t2 are the migration times of isomer 1 and 2 and W1 and W2 are the peak widths at baseline of each isomer. 1: (6R,2'S)-diastereomer 2: levo-folinic acidBGE: 6.0 mmol/L PIP-β-CD, 30 mmol/L phosphate buffer containing 10% acetonitrile, 20 kVThe effect of the pH on time of EOF (tEOF), the migration time (t), resolution (Rs) and separation selectivity (α) of folinic acid diastereomers.