CENP-A mRNA and protein expression in HCC cell lines and tumor tissues.

A: RT-PCR (left) and qPCR (right) analysis of CENP-A mRNA expression in two HCC cell lines HepG2 and SMMC-7721. B: Western blotting analysis of CENP-A protein product in HepG2 and SMMC-7721 cells. Left: Representative Western blots of three independent experiments. Right: Quantification of the CENP-A Western blots. C: RT-PCR (upper) and qPCR (lower) analysis of CENP-A transcript in HCC tumor (T) and corresponding nontumorous (N) liver tissues. D: Western blotting analysis of CENP-A protein expression in HCC and adjacent nontumor tissues. The bar graph below the Western blot panels shows the quantification of the protein level of CENP-A. GAPDH was used as an internal control in all the experiments. **Pt-test; n = 3 in A and B, and n = 20 in C and D.