Amino acids encoded at polymorphic codons of 6 P. falciparum loci likely to be under drug selection.

Genome sequence data was generated on the Solexa Illumina platform as described in Materials and Methods. Sequences aligning with the 6 loci shown in the reference sequence for P. falciparum (laboratory clone 3D7) were extracted (with quality criteria stated in the Methods), converted to FASTA format, translated and aligned in Clustal W. Sites exhibiting polymorphism among the 5 isolates are shown.Shading: non-synonymous substitution relative to the reference sequence.Multiplicity: where more than one base was called at any one position, the encoded amino acid with the most calls is displayed above. Haplotypes cannot be inferred by these data – for example any or all combinations of YFY, YFD, NFY or NFD may exist for pfmdr1 codons 86, 184 and 1246 in patient OX001.