RNA-seq of NFATC1's role in AhBMSCs

We report the application of RNA sequencing technology for high-throughput profiling of gene expression in NFATC1 knockdown (siNFATC1) or inactivation (FK506 treatment) adult human bone marrow mesenchymal stem cells (AhBMSCs).To knockdown NFATC1 in AhBMSCs we transfected cells with NFATC1 specific siRNAs and the scrambled RNAs were ste as controls. To block NFATC1 activation we used 20µM FK506 and its control was DMSO. AhBMSCs are derived from a 24-year-old healthy male human donor. Total mRNA was collected using TRIzol ® (Invitrogen, CA, USA) solution. Then all the extraction procedures were conducted according to manufacture's instructions for RNA extraction from homogenized tissues. After quality checks they were subjected to the high-throughput RNA-sequencing. Every group consists of 3 replicates which were from 3 independent experimental repeats. Overall design: Examination of RNA expression in AhBMSCs after NFATC1 knockdown or FK506 treatment respectively.