native top-down MS data of human protamines collected on orbitrap fusion lumos. Samples were collected from different patient populations, namely normal, asthenozoospermia, and oliogoasthenozoospermia
A proper internal standard choice is critical for accurate, precise, and reproducible mass spectrometry-based proteomics assays. Synthetic isotopically labeled (SIL) proteins are currently considered
Spectral library search can enable more sensitive peptide identification in tandem mass spectrometry experiments. However, its drawbacks are the limited availability of high-quality libraries and the