Solid phase extraction and liquid/liquid extraction of MC252 water soluble compounds produced by photodegradation and biodegradation characterized by FT-ICR mass spectrometry

Two widely used extraction techniques, liquid/liquid extraction, and solid-phase extraction were applied with styrene-divinylbenzene (SDVB) polymer with a proprietary non-polar surface (Priority Pollutant, PPL), to water-soluble compounds generated through photodegradation and biodegradation of petroleum. The molecular composition of biodegraded and photo-degraded water-soluble organic acids was compared to identify analytical biases and compositional differences in these two widely used fractionation techniques by 21 tesla negative-ion electrospray ionization FT-ICR mass spectrometry (MS). The compositional differences between the two extraction techniques for abiotic and biotic degradation process were highlighted. Toxic species (naphthenic acids) produced through hydrocarbon biodegradation identified by LLE that are not recovered with SPE extraction of the same water sample were identified.