Quantitative results corresponding to the work "Novel genipin-crosslinked acellular biogenic conduits for tissue engineering applications"

This dataset corresponds to the quantification results carried out for the biogenic conduits generated and several technical controls analyzed in the manuscript entitled "Novel genipin-crosslinked acellular biogenic conduits for tissue engineering applications". Tissue engineering has developed and analyzed a wide range of bioengineered conduits made from synthetic or natural material for different clinical applications. Though, replicating the extracellular matrix's (ECM) structural and biochemical complexity is challenging with conventional biofabrication procedures. Consequently, researchers have generated collagen-based conduits or membranes in vivo by stimulating a fibrotic response through the implantation of a non-resorbable material in animal models. This study is the first one to characterize ex vivo the biogenic conduits of 1- and 2-months maturation time which were subjected to decellularization and genipin (GP) crosslinking procedures performing histological, structural, biomechanical, biocompatibility, and immunological analyses to identify the most suitable option for future peripheral nerve regeneration research. Results: Histological examination indicated consistent uniformity in the bioconduits at both 1- and 2-months post-implantation, maintaining their overall structural integrity and collagen pattern following decellularization and GP crosslinking treatments. Furthermore, there was no evidence of nuclear debris observed in the decellularized groups at either stage of maturation, confirming the decellularization protocol's efficiency. The substitutes with longer maturation time presented a generally higher preservation of ECM key components. In addition, the GP crosslinking revealed significantly increased resistance values of decellularized bioconduits in the tensile test, without affecting drastically the ex vivo cell biocompatibility nor macrophage polarization rate phenotype. The original data obtained for the quantitative analyses of the different biogenic conduits generated are shown in this dataset.