The effect of cold pre-treatment on survival of Drosophila melanogaster larvae after the acute cold shock (CS) to -12°C for 1 hour.

1 Acute pre-treatment, 1 h at -4°C; chronic pre-treatment, 1.25 d at 0°C. Both pre-treatments were followed by recovery at 18°C for 2 h.2 Statistical significance of the difference between control (no pre-treatment) and pre-treated larvae was tested using Fishers' exact test. Significant differences are shown in bold letters:**, P ***, P 3 The relative risk of death expresses the chance of death in control larvae (not pre-treated) in comparison to pre-treated larvae.The effect of cold pre-treatment on survival of Drosophila melanogaster larvae after the acute cold shock (CS) to -12°C for 1 hour.