Lysine Deprivation Suppresses Adipogenesis in 3T3-L1 Cells: A Transcriptome Analysis

Organism: Mus musculus Summary: It has been reported that low concentration of lysine suppressed lipid accumulation and transcription of several adipogenic genes in 3T3-L1 preadipocytes, however, the detailed lysine deprivation-specific cellular transcriptomic changes and the altered pathways have not yet been fully decrypted. Overall design: Using 3T3-L1 cells, we performed RNA sequencing on undifferentiated cells, differentiated cells, and differentiated cells under a lysine-free culture medium

物种：小鼠（Mus musculus）。摘要：研究表明，赖氨酸的低浓度水平可抑制3T3-L1前脂肪细胞中脂质积累及多种脂肪生成基因的转录，然而，赖氨酸剥夺所特有的细胞转录组变化及其改变的通路尚待全面解析。总体设计：本研究采用3T3-L1细胞，对未分化细胞、分化细胞以及赖氨酸缺乏的培养介质中的分化细胞进行了RNA测序。