Molecular consequences of Dlx3 deletion in mouse enamel organ as determined by RNASeq analysis

DLX3 is a homeodomain transcription factor involved in ameloblast differentiation and enamel formation. Mutations in DLX3 in human lead to defects in enamel. However, the downstream targets of Dlx3 transcriptional activity in the enamel organ have not been identified yet. In this study, we compared the transcriptome of enamel organs where Dlx3 has been deleted to control tissues. Total RNA was extracted from enamel organs (mandibular incisors) from Dlx3-WT (N=4) and Dlx3-K14cKO (N=4) mice at P10. cDNA libraries were generated using NEBnext and NuGen kits. Sequencing was performed on the HiSeq2000.DLX3是一种同源域转录因子（homeodomain transcription factor），参与成釉细胞分化与牙釉质形成。人类DLX3基因发生突变可引发牙釉质发育缺陷。然而，目前尚未明确牙釉质器官中Dlx3转录活性的下游靶标基因。本研究对Dlx3敲除的牙釉质器官与对照组织的转录组开展比对分析：研究人员从出生后第10天（P10）的Dlx3野生型（Dlx3-WT，N=4）与Dlx3-K14条件性敲除（Dlx3-K14cKO，N=4）小鼠的下颌切牙来源的牙釉质器官中提取总RNA，使用NEBnext与NuGen试剂盒构建cDNA文库，随后在HiSeq2000测序平台上完成测序。