Figure_4_images_used_for_paper

(B, F, G) HEK293T ishXrn1cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with luciferase reporters containing insertions of the indicated lengths from the YKT6 or STOML2 genes (B). In F and G, constructs also carry mutations that disrupt the hairpin structure and restore with a different sequence. PR8 PA-X or an empty vector were also co-transfected. RNA was extracted to run 5’ RACE. DNA bands were purified and sequenced to confirm their identities. Gel images representative of 3 experiments. Additional replicates are located in Figure_4_replicates dataset.

R# --> replicate number; AI#/LG### --> experiment number.

.sgd are the original source files acquired with the Syngene Imager.