Supplemental Material for Colonnetta, et al. 2021
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<b>Figure S1. Examples of defect classes for analysis of patterning gene, mitotic, and cytoskeletal phenotypes.</b><b><br></b><b>Figure S2. Zygotic knockdown of CLAMP depletes protein levels in early embryonic nuclei.</b><b><br></b><b>Figure S3. Zygotic knockdown of Bicoid depletes protein levels in the anterior of <i>bcdi</i> embryos.</b><b><br></b><b>Figure S4. RNA-seq reveals more decreased targets in early vs. late datasets.</b><b><br></b><b>Figure S5. Localization of CLAMP and Zld in genomic regions containing the pair-rule genes <i>eve</i> and <i>run</i> and the gap genes <i>btd</i> and <i>gt</i>.</b><b><br></b><b>Figure S6. Levels of CLAMP protein levels and patterning gene proteins correlate in <i>clampi</i> embryos.</b><b><br></b><b>Figure S7. Transcription of the pair-rule gene<i> runt </i>is disrupted in <i>clampi<sup>1</sup></i> or <i>zldi </i>embryos.</b><b><br></b><b>Figure S8. Zygotic <i>clamp</i> knockdown results in variable decrease in Buttonhead protein levels.</b><b><br></b><b>Figure S9. CLAMP and Zelda are required for proper expression of the gap gene<i> gt</i>.</b><b><br></b><b>Figure S10. Levels of Sxl protein are decreased in female compromised for CLAMP activity.</b><b><br></b><b>Figure S11. CLAMP occupancy shifts during the minor wave of ZGA.</b><b><br></b><b>Table S1. Decreased targets in both CLAMP knockdown collections at 0-1.5 hr and 1.5-3 hr. </b> <b>Table S2. Comparison of decreased zygotic genes between previously published datasets and our RNA-seq data from zygotic knockdown embryos. </b> <b>Table S3. Maternal/zygotic classification of target genes decreased in 0-1.5 hr RNA-seq datasets. </b> <b> </b> <b>Table S4. CLAMP binding genes near the TSS increases at NC11 and is maintained throughout later nuclear cycles. </b> <b>Table S5. Decreased transcripts (by at least 2-fold) in 0-1.5 hr collections of <i>clampi<sup>1</sup></i>, <i>zldi</i>, and <i>zld/clampi<sup>1</sup></i> embryos.</b><b><br></b><b>Reagent Table details all reagents used in the manuscript. </b><b><br></b><b>Colonnetta et al. CLAMP_Genetics_Supplement contains full figure legends. </b>
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GSA Journals
创建时间：
2021-06-23
