Data from: An opponent striatal circuit for distributional reinforcement learning

We trained thirsty mice in a classical conditioning task in which random
odors were paired with different probability distributions over water
amounts. Behavior was monitored using an IR lick sensor, a face camera,
and a body camera, and non-licking behavioral variables were extracted
using Facemap. We recorded from neurons in the anterior striatum using
acute Neuropixels probes and registered each cell to the Allen Common
Coordinate Framework. In a subset of mice, we injected 6-hydroxydopamine
unilaterally into the lateral nucleus accumbens shell (lAcbSh) before
training to lesion dopamine axons projecting to this region, and then
recorded after training. Separately, we recorded specifically from lAcbSh
neurons expressing either the D1 or D2 dopamine receptor using two-photon
calcium imaging in transgenic mice. Finally, we expressed excitatory
(CoChR) or inhibitory (GtACR1) opsins in these cell types and measured
changes in licking induced by optogenetic stimulation.