Gene expression profile at single cell level of the lung tissue of the septic mice

Single-cell sequencing of mouse lung tissue from the Sham group, CLP group, CLP+Maresin1 and the CLP-48h group. We used the Cecal ligation and puncture (CLP), the most commonly used method to induce polymicrobial sepsis, to construct the mouse model of septic lung injury. The mice in Sham group , CLP group,CLP+Maresin1 group were sacrificed 24 hours after operation, while the CLP-48h group were sacrificed at 48 hours after operation.The mice in the CLP+Maresin1 group were administered 1 ng of Maresin1 via the tail vein. The lung tissue samples were collected for subsequent testing and analysis. Three sham group specimens, three CLP specimens, three CLP-48h specimens and three CLP+Maresin1 group specimens were mixed for single-cell RNA sequencing. The prepared single cell suspensions was subjected to Magnetic-activated cell sorting (MACS) using Miltenyi mouse CD45 magnetic beads according to the manufacturer's recommendations. The CD45- and CD45+ cell suspensions were mixed 1:1 for single-cell sequencing.