Setd2 kidney conditional knockout mouce

SET domain-containing 2 (SETD2) is the primary methyltransferase for histone 3 lysine-36 trimethylation (H3K36me3) and SETD2-mediated H3K36me3 is involved in the regulation of alternative splicing, mRNA methylation, and DNA mismatch repair in mammalian cells. SETD2 has been shown as one of the most important tumor suppressors in renal cancers. To explore a physiological role for SETD2 in renal tubule, we inactivated Setd2 specifically in mouse kidney using PAX8-Cre transgenic mouse and analyzed this mouse model using metabolic chamber. We also conducted RNA sequencing to decipher the underlying molecular mechanism determining metabolic phenotypes of Setd2-deficienty kidney. The mice were 10-week old and six samples each of control mouse and transgenic mouse were used.