Analysis of the antifungal activity and bioactivity of gold nanoparticles conjugated with Os-C(W5)

These datasets consist of quantitative and qualitative data from the analysis of GNP@Os-C(W5) including spectrophotometry readings, scanning electron microscopy images and circular dichroism findings. A 535 nm band and GNPs of 13.2 ± 2.4 nm confirmed the successful synthesis of GNP@Os-C(W5). Circular dichroism identified that the secondary structure was disordered, similar to Os-C(W5). Anticandidal activity was reduced with a 50% minimum inhibitory concentration (MIC50) of 13.21 ± 0.148 μM for GNP@Os-C(W5) compared with 1.37 ± 0.076 μM for Os-C(W5) under the conditions used in this study. Additionally, the minimum fungicidal concentrations were 10 μM for Os-C(W5) and 34 μM for GNP@Os-C(W5) where the two were identified as fungistatic and fungicidal respectively. At the respective MIC50 values, both compounds caused membrane associated cellular damage. Furthermore, both were non-cytotoxic at 1.37 μM Os-C(W5) and 6.84 μM GNP@Os-C(W5) in human erythrocytes and leukocytes. For GNP@Os-C(W5) compared with Os-C(W5), antioxidant activity was reduced by several fold. Although the anticandidal activity of GNP@Os-C(W5) was reduced, the GNP conjugate showed greater resistance to trypsin degradation than melittin and Os-C(W5).

本数据集汇聚了GNP@Os-C(W5)分析中所得的定量与定性数据，包括光谱光度计读数、扫描电子显微镜图像以及圆二色谱检测结果。535纳米波段及13.2 ± 2.4纳米的GNPs证实了GNP@Os-C(W5)的成功合成。圆二色谱分析显示，其二级结构无序，与Os-C(W5)相似。在研究条件下，与Os-C(W5)的1.37 ± 0.076 μM最低抑菌浓度（MIC50）相比，GNP@Os-C(W5)的最低抑菌浓度（MIC50）为13.21 ± 0.148 μM，抗念珠菌活性有所下降。最低杀菌浓度分别为Os-C(W5)的10 μM和GNP@Os-C(W5)的34 μM，其中两者分别被鉴定为抑菌和杀菌。在各自的MIC50值下，两种化合物均引起与膜相关的细胞损伤。此外，在1.37 μM的Os-C(W5)和6.84 μM的GNP@Os-C(W5)下，两者对人类红细胞和白细胞均未表现出细胞毒性。与Os-C(W5)相比，GNP@Os-C(W5)的抗氧化活性降低了数倍。尽管GNP@Os-C(W5)的抗念珠菌活性有所降低，但该金纳米颗粒共轭物对胰蛋白酶降解的抵抗力强于蜂毒素和Os-C(W5)。