Methionine biosynthetic pathway genes metH and metZ affect curdlan synthesis in Agrobacterium sp. CGMCC 11546

Purpose: The goal of this study is compare the effect of MetH and MetZ gene in curdlan synthesis in Agrobacterium sp. CGMCC 11546. methods: The transcriptional and metabolomics analysis the function of metH and metZ in Agrobacterium sp. CGMCC 11546. Results: The transcriptional and metabolomics showed that the decrease of curdlan production in the ΔmetH and ΔmetZ mutants may be caused by the insufficient supply of energy ATP conclusion: MetH and MetZ play an important role in curdlan synthesis in Agrobacterium sp. CGMCC 11546 The total RNA of each sample from the wild type strain and the gene deletion mutants after 24 hours fermentation, samples from metH deletion mutant named metH-1, metH-2 and metH-3; three biological repeats. samples from metZ deletion mutant named metZ-1, metZ-2 and metZ-3; three biological repeats; samples from the wild type strain named WT-1, WT-2 and WT-3; three biological repeats