Overlap of cargo binding sites on myosin V coordinates the inheritance of diverse cargoes
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https://www.omicsdi.org/dataset/bioimages/S-JCBD-201201024
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Figure 4, X. A Myo2 mutation that disrupts Vac17 but not Mmr1 binding exhibits a defect in vacuole inheritance. myo2∆ cells containing myo2-D1297N on a plasmid were transformed with pmitoGFP (green) and grown in synthetic complete minus leucine media for at least six doubling times, keeping the density < 5x106 cells/ml. Cells were then pulsed with FM4-64 for 1 hr and chased for one doubling time before adsorbing to ConA-labeled glass chambers. Red, vacuoles; green, mitochondria; grey, DIC. 1563 s
创建时间：
2018-11-29
