PI3Kg in B cells promotes antibody responses and generation of antibody-secreting cells [RNA-seq]

The differentiation of naïve and memory B cells into antibody-secreting cells (ASCs) is a key feature of adaptive immunity. The requirement for phosphoinositide 3-kinase delta (PI3Kd) to support B cell biology has been investigated intensively; however, specific functions of the related phosphoinositide 3-kinase-gamma (PI3K?) complex in B-lineage cells have not. Here we report that PI3K? promotes robust antibody responses induced by T cell-dependent antigens. The inborn error of immunity caused by human deficiency in PI3K? results in broad humoral defects, prompting our investigation of roles for this kinase in antibody responses using mouse models with bulk and single-cell transcriptome as well as B cell receptor repertoire sequencing. Overall design: For bulk RNA sequencing, we immunized mice (3 wild-type and 4 Pik3cg knockout) with sheeps red blood cells via IP injection. At day 28 we harvested various tissues and sorted cells via FACS. Total RNA was submitted to the Yale Center for Genome Analysis for sequencing.