ena-DATASET-dkfz_b062-15-06-2020-17:38:56:503-456 - samples
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下载链接：
https://www.omicsdi.org/dataset/ega/EGAD00001006218
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资源简介：
This dataset contains miRNA-seq data from 10 patients.
Small RNAs were isolated as described previously57,58 from fresh-frozen
tumour material. In brief, total RNA was extracted using guanidinium
isothiocyanate/phenol extraction followed by 3′-adaptor ligation of
barcoded adenylated adaptors. Samples were pooled in two sets of five
samples. Subsequently, gel electrophoresis was used to isolate small
RNAs (19–35 nt) and purified using ethanol precipitation. Fragments
were then amplified using standard PCR, isolated using gel electropho-
resis and purified using ethanol precipitation. Samples were sequenced
on a HiSeq 2000 v.4 machine resulting in 10 Fastq files.EGA dataset EGAD00001006218
创建时间：
2020-07-16
