Reovirus infection induces stabilization and up-regulation of cellular transcripts that encode regulators of TGF-beta signaling

We utilized oligonucleotide microarrays to measure cellular mRNA decay rates in mock- or reovirus-infected murine L929 cells to determine if changes in host mRNA expression are a consequence of reovirus-induced alterations in cellular mRNA stability. Murine L929 cells were mock-infected or infected with reovirus strains clone87, clone8, and Dearing. At 19.5 h p.i., ongoing transcription was arrested by addition of actinomycin D and global mRNA expression levels were measured after 0, 45, 90 and 120 min of actinomycin D treatment using Affymetrix U74Av2 microarrays