miRNA profile of young mice and aged mice

Paternal aging has been reported to be related with offspring`s increased risk of autism spectrum disorders. However, effect of miRNAs in sperm has not been investigated. We used microarray to investigate whether miRNA profile were changed with aging. Sperm from mice at 3 months old (3M), >12M and >20M were collected and miRNAs of the sperm were extracted. Then, we conducted microarray analyses on the extracted miRNAs.