Neuron-astrocyte metabolic coupling protects against activity-induced fatty acid toxicity

Gene expression profiling of cultured glia with low or high levels of lipid droplets We stained lipid droplets (LDs) in cultured rat glia with nile red stain. We seperated glia containing high numbers of LDs using fluorescence activated cell sorting (FACS). We performed mRNA-seq experiments on glia with low or high levels of LDs.