Effect of the application of the formulation of Pseudomonas chlororaphis PCL1606 in the prokaryotic and eukaryotic communities present in soil and rhizosphere samples taken from avocado plants

In this work, a semi-commercial formulated product based on
PcPCL1606 was used to study the impact of the application of PcPCL1606 on
natural microbial populations. The treatment was a preventive application
(PcPCL1606 preventive), consisting of a single application with a
semi-commercial formulate of PcPCL1606 (PcPCL1606 preventive). To test the
effect of the preventive PcPCL1606 application in the microbiome avocado,
samples from T3 of assay 2 (130 days after inoculating) in the control and
preventive treatment were analysed. DNA was extracted from each of the 3
independent composite samples where formulated PcPCL1606 was applied (PcPCL1606
preventive) and 3 composite samples where it was not applied (Control). DNA
samples taken from each rhizosphere/soil type were sent to be sequenced by
ChunLab (Seoul, Korea) to obtain the microbial DNA sequences of the 16S rRNA
gene and ITS hypervariable regions.