Effect of the application of the formulation of Pseudomonas chlororaphis PCL1606 in the prokaryotic and eukaryotic communities present in soil and rhizosphere samples taken from avocado plants

In this work, a semi-commercial formulated product based on PcPCL1606 was used to study the impact of the application of PcPCL1606 on natural microbial populations. The treatment was a preventive application (PcPCL1606 preventive), consisting of a single application with a semi-commercial formulate of PcPCL1606 (PcPCL1606 preventive). To test the effect of the preventive PcPCL1606 application in the microbiome avocado, samples from T3 of assay 2 (130 days after inoculating) in the control and preventive treatment were analysed. DNA was extracted from each of the 3 independent composite samples where formulated PcPCL1606 was applied (PcPCL1606 preventive) and 3 composite samples where it was not applied (Control). DNA samples taken from each rhizosphere/soil type were sent to be sequenced by ChunLab (Seoul, Korea) to obtain the microbial DNA sequences of the 16S rRNA gene and ITS hypervariable regions.