Space Associated Stem Cell Hallmarks of Aging in Astronauts

This study examined the effects of the stress of space, specifically low earth orbit, on hematopoietic stem and progenitor cells (HSPCs) that maintain lifelong hematopoiesis and immune responses. Enriched CD34+ HSPCs were derived from 9 astronauts before, during and after 10 and 21-day ISS missions on the International Space Station. The samples were used as input to whole transcriptome sequencing by bulk RNA-Seq performed at The Scripps Research Institute Next Generation Sequencing Core on an Illumina NextSeq 2000 sequencer, single cell RNA-Seq performed at The Salk Institute for Biological Studies on Illumina NovaSeq X Plus sequencer, and whole genome sequencing at The Scripps Research Institute Next Generation Sequencing Core on an Illumina NovaSeq 6000 sequencer. Subsequent... (for more see dbGaP study page.)