Controlling and Measuring the Equilibration of Dynamic Combinatorial Libraries of Imines

Imine formation is one of the most powerful methods for the construction of azamacrocycles, especially when combined with Dynamic Combinatorial Chemistry. Careful NaBH4 reduction of the imine libraries opens the possibility of determination of their composition via HPLC analysis of the resulting amine mixture. Templates, reagent concentrations, as well as reaction conditions influence the library composition.