Gliopathic Bang Sensitive Siezure Common Expression

In order to identify common gene expression across other gliopathic bang senstiive genes (i.e. genes that when knocked down or over expressed by repo-GAL4 cause seizures) we crossed six UAS-RNAi or UAS-over-expression lines for known bang senstive genes to repo-GAL4. We pooled total RNA from brains of these animals in equal amounts and ran microarray analysis to search for transcripts that are common among these six seizure associated genes. Controls are empty TRiP RNAi vector crossed to repo-GAL4. Experimental pool is SesB-RNAi; COX7A-RNAi; UAS-Dube3a; UAS-htt.12Q.FL; comt-RNAi; nrv2-RNAi and ATPalpha-RNAi crossed to repo-GAL4.