Expression data from Hela cells and HSF2-deficient HeLa cells subjected to heat treatment in the presence of IHSF115, an inhibitor of HSF1.

The majority of heat-induced genes were inhibited by IHSF115, i.e. were positively regulated by HSF1, suggesting that HSF1 plays a predominant role in the transcription of heat-induced genes IHSF115 effectively countermanded repression in a significant fraction of heat-repressed genes, suggesting that repression of these genes is mediated by transcriptionally active HSF1. Transcriptomic changes in cells incubated for 2h at 37ºC with vehicle (DMSO) or IHSF115, subjected to heat treatment at 43ºC for 30min in a thermostatically controlled water bath and then incubated for an additional 1h at 37ºC. The assays were conducted by triplicate (HeLa cells) or quintuplicate (HSF2-deficient Hela cells).