CCL28 modulates neutrophil responses during infection with mucosal pathogens

The chemokine CCL28 is highly expressed in mucosal tissues, but its role during infection is not well understood. Here we show that CCL28 promotes neutrophil accumulation in the gut of mice infected with Salmonella and in the lung of mice infected with Acinetobacter. Neutrophils isolated from the infected mucosa expressed the CCL28 receptors CCR3 and, to a lesser extent, CCR10, on their surface. The functional consequences of CCL28 deficiency varied between the two infections: Ccl28-/- mice were highly susceptible to Salmonella gut infection but highly resistant to otherwise lethal Acinetobacter lung infection. In vitro, unstimulated neutrophils harbored pre-formed intracellular CCR3 that was rapidly mobilized to the cell surface following phagocytosis or inflammatory stimuli. Moreover, CCL28 stimulation enhanced neutrophil antimicrobial activity, production of reactive oxygen species, and formation of extracellular traps, all processes largely dependent on CCR3. Consistent with the dif..., See manuscript materials and methods for details, , # CCL28 Manuscript Source Data File

[https://doi.org/10.5061/dryad.59zw3r2j6](https://doi.org/10.5061/dryad.59zw3r2j6)

## Description of the data and file structure

These are source data files for the manuscript: CCL28 modulates neutrophil responses during infection with mucosal pathogens. eLife (2024). Walker, Perez-Lopez, et al.

Data is contained in files in accordance to the respective manuscript figure names. Each file contains data from separate panels in each figure on separate tabs.

The materials and methods and the figure legends contain all information needed for analysis of each data file.

Further description of individual files and tabs:

### Files and variables

#### File: elife\_CCL28\_Figure1\_SourceData.xlsx

**Description**: File contains data from mice gavaged with streptomycin 24h prior to oral infection with approximately 10^9 CFU S. enterica serovar Typhimurium (STm).

A) At 4 days post-infection (dpi), CCL28 in feces was quantified by ELISA. Data comprise two ...