Confocal and micro-CT data of Rutrizoma spp.

This dataset contains the raw confocal laser scanning microscopy (CLSM) slices and X-ray microtomography (micro-CT) slices for Rutrizoma donoghuei (NIGP166143, NIGP166142, NIGP205681, NIGP205682-1, NIGP205683 and NIGP205684), Rutrizoma pisanii (NIGP205685-1), and unidentified specimens of Rutrizoma (NIGP205686-1 and NIGP205688). This dataset is associated with the publication "Specialized bark-gnawing beetles reveal phragmotic defence and subcortical ecology in the Cretaceous" (DOI: 10.1098/rspb.2025.1004). Confocal images were obtained with a Zeiss LSM710 confocal laser scanning microscope, using the 561 nm (DPSS 561-10) laser excitation line. The original CZI files are provided, which could be opened by the ZEISS ZEN software. Micro-CT data were obtained with a Zeiss Xradia 520 Versa 3D X-ray microscope. Scanning parameters were as follows: NIGP166143 [isotropic voxel size, 4.7892 μm; power, 3 W; acceleration voltage, 40 kV; exposure time, 5 s; projections, 3001]; NIGP205681 [isotropic voxel size, 4.6020 μm; power, 3 W; acceleration voltage, 40 kV; exposure time, 2.5 s; projections, 2501]; NIGP205685-1 [isotropic voxel size, 7.8749 μm; power, 4 W; acceleration voltage, 50 kV; exposure time, 2 s; projections, 2201]; NIGP205688 [isotropic voxel size, 4.4238 μm; power, 4 W; acceleration voltage, 50 kV; exposure time, 1 s; projections, 2501]. The TIFF stacks are provided.