Serological and molecular detection of Leptospira spp in dogs

Abstract INTRODUCTION: This study aimed to detect anti-Leptospira spp antibodies and Leptospira DNA in domestic dogs. METHODS: Blood and urine from 106 dogs were evaluated by microscopic agglutination test (MAT) and polymerase chain reaction (PCR), respectively. RESULTS: Six (5.7%) and one (1%) animals were positive by MAT and PCR, respectively. CONCLUSIONS: These results show a low prevalence of infection by Leptospira spp. The absence of positive results for the Icterohaemorrhagiae serogroup indicates the small relevance of these dogs as sources of human leptospirosis.

摘要 引言：本研究旨在检测家养犬体内的抗钩端螺旋体属（Leptospira spp.）抗体及钩端螺旋体DNA。
方法：本研究分别采用显微凝集试验（microscopic agglutination test, MAT）与聚合酶链式反应（polymerase chain reaction, PCR），对106只家养犬的血液与尿液样本进行检测。
结果：经检测，6只（占比5.7%）犬的样本在MAT检测中呈阳性，1只（占比1%）犬的样本在PCR检测中呈阳性。
结论：本研究结果显示，家养犬钩端螺旋体属感染的流行率较低；未检测到黄疸出血群血清型（Icterohaemorrhagiae serogroup）的阳性样本，提示此类犬作为人类钩端螺旋体病传染源的相关性有限。