Blastocysts derived from 8-cell stage embryos which were developed with 1% follicular fluid.

Follicular fluid (FF) is a potential environment for early embryonic development because FF fluxes into the oviduct at ovulation. This data is RNA-seq of blastocyst stage embryos developed from 8-cell stage embryos which were developed with 1% FF (treatment period: from pronuclear stage, 18h post insemination, to 8-cell stage embryos, 48h pose insemination). Oocyte-cumulus cells complexes (COCs) were collected from antral follicles of Japanese Black Cows-ovaries collected at a slaughter house and cultured in TCM-199 medium 5mM taurine, 10% FCS and 10ng/ml EGF. At the end of culture period (21hrs), oocytes were fertilized with frozen semen of a Japanese Black Bull. After sperm and COCs incubation (6h), zygotes were denuded from the cumulus cells and incubated in synthetic oviductal fluid medium containing 0 or 1% FF and 1% FCS. Blastocyst stage embryos were obtained at days after insemination and the embryos were subjected to RNA-seq. RNA extraction was conducted using RNAqueous Total RNA Isolation Kit (Invitrogen). Three samples were prepared from differential ovary series. The RNA quality and concentration were examined using a Agilent Bioanalyzer 2100 (Agilent technologies). cDNA of the embryos was produced from each RNA using NEBNext Single Cell/Low Input RNA Library Prep Kit (New England). The quality and quantity were determined using the Agilent Bioanalyzer 2100, followed by re-measurement using Kapa Library Quantification Kit Kapa Biosystems. Sequencing was conducted using NextSeq1000 Single read x 100 bp. Image analysis, base calling and quality filtering were performed using the RTA version 2.4.11 (Illumina) following the manufacturer's instructions, and the sequence data was converted to Fastq using bcl2fastq2 v2.20.0.422.