Guisoni2016 - Cis-regulatory system (CRS) can drive sustained oscillations

Guisoni2016 - Cis-regulatory system (CRS) can drive sustained oscillations This model is described in the article: Promoters Architecture-Based Mechanism for Noise-Induced Oscillations in a Single-Gene Circuit. Guisoni N, Monteoliva D, Diambra L. PLoS ONE 2016; 11(3): e0151086 Abstract: It is well known that single-gene circuits with negative feedback loop can lead to oscillatory gene expression when they operate with time delay. In order to generate these oscillations many processes can contribute to properly timing such delay. Here we show that the time delay coming from the transitions between internal states of the cis-regulatory system (CRS) can drive sustained oscillations in an auto-repressive single-gene circuit operating in a small volume like a cell. We found that the cooperative binding of repressor molecules is not mandatory for a oscillatory behavior if there are enough binding sites in the CRS. These oscillations depend on an adequate balance between the CRS kinetic, and the synthesis/degradation rates of repressor molecules. This finding suggest that the multi-site CRS architecture can play a key role for oscillatory behavior of gene expression. Finally, our results can also help to synthetic biologists on the design of the promoters architecture for new genetic oscillatory circuits. This model is hosted on BioModels Database and identified by: MODEL1611030000. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.