Determination of MHV virion genome poly(A) tail length and 3' end additions.
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The mouse hepatitis virus (MHV) genomic RNA has a poly(A) tail required for replication. Here we investigated the presence of terminal poly(A) tail uridylation and guanylation in the virion RNA. After isolating the viral RNA followed by sequencing, we found a mean poly(A) tail of 66 nucleotides long with a peak of terminal uridylation on tails 55 to 66 nucleotides long. Direct RNAseq of RNA isolated from MHV virion
创建时间：
2023-05-12
