Functionally distinct promoter classes initiate transcription via different mechanisms [PRO-Seq]
收藏NIAID Data Ecosystem2026-05-01 收录
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To identify the functional contribution to transcription of various promoter-bound proteins we have endogenously tagged select proteins with an auxin-inducible degron and measured nascent transcription with PRO-seq 6 hours after their depletion Endogenously tagged AID cell lines were created using CRISPR/Cas9 in Drosophila S2 cells constitutively expressing OsTir ligase. PRO-seq was performed 3, 6 or 12 hours after auxin addition
创建时间：
2023-06-01
