Chromatin accessibility landscapes across T-ALL

Acute lymphoblastic leukemia (ALL) is the most common malignancy in pediatric patients and represents about a quarter of all pediatric malignancies. While transcriptional and DNA methylation profiling of these subtypes has been extensively examined, the accompanying chromatin landscape and corresponding gene regulatory repertoire is not well characterized for many ALL types. To better understand the ALL epigenome and gene regulatory network we mapped chromatin accessibility for T-ALL patient samples using assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) . Overall design: Fast-ATAC-seq was performed on purified leukemia cells from human patients. 10,000 cells were used for library preparation.