Transcriptomics study on HepG2 cells treated with different doses of caffeine for 24 h.

With the general adoption of new approach methodologies, the omic-based technologies are of particular importance for chemical hazard characterization owning to the premise that any apical endpoint change indicative of impaired health must be underpinned by some alterations at the omic level. In this work we studied cellular response to caffeine by measuring transcriptomics experiments. The HepG2 cells were treated with 6 doses of caffeine for 24 h. 1 control group was set (0 uM of coumarin). Each group has 3 biological replicates. The HepG2 cells were treated with 6 doses of caffeine for 24 h. 1 control group was set (0 uM of caffeine). Each group has 3 biological replicates (21 samples in total).