Figure S1 - Identification of B Cell Epitopes of Alcohol Dehydrogenase Allergen of <em>Curvularia lunata</em>

Sequence alignment of CADH with ADH from different sources. The sequences of alcohol dehydrogenase from various sources were aligned using Clustal W (1.81) multiple sequence alignment tool (http://align.genome.jp/) and manually optimized. The sequences are as follows: 1) Chain A of human alpha alcohol dehydrogenase, 2) Chain A, human beta-1 alcohol dehydrogenase, 3) Alcohol dehydrogenase E chain from Equus caballus (horse), 4) alcohol dehydrogenase from Rattus norvegicus (Norway rat), 5) Chain A of mouse alcohol dehydrogenase from Mus musculus, 6) alcohol dehydrogenase 1 from Zea mays, 7) alcohol dehydrogenase from Arabidopsis thaliana, 8) alcohol dehydrogenase II from Candida albicans, 9) alcohol dehydrogenase from Candida albicans, 10) alcohol dehydrogenase from Saccharomyces cerevisiae, 11) alcohol dehydrogenase from Cochliobolous lunatus, 12) alcohol dehydrogenase from Schizosaccharomyces pombe. Curvularia lunata ADH (CADH) accession number is highlighted in blue whereas the conserved residues are denoted by an asterisk (*). CADH sequence shows the presence of all 22 residues conserved across kingdoms as reported by Jornvall et al., 1987 (40). These residues are highlighted in yellow. (DOCX)