Kinetic data for the refolding/unfolding of wt-ub and (4R)-FPro-ub monitored by changes in Tyr fluorescence using GdmCl denaturant.

Experiments were performed in 10 mM Gly/HCl buffer at pH 2.0 and at 25°C. Rate-constants and m-values listed were determined by globally fitting folding/unfolding rate constants and equilibrium fluorescence signals to a three-state on-pathway mechanism as described in material and methods. aRate constants have units s−1. bm-values have units kJ·mol−1·M−1. cΔGun calculated using −RT ln(Kui(kin/kni)); units are kJ·mol−1. dαTS1 is defined as mui/(mui+miu). eαTS2 is defined as min/(min+mni).