Metagenomics analysis of bacterial diversity present in homemade formulation of probiotics applied to Biofloc Aquaculture systems

Biofloc fish farming is based on in situ microorganism production, which maintainswater quality, generates microbial protein, and outcompetes potential pathogens. Anexogenous application of probiotics will provide promising results to bioflocaquaculture, but relatively less number of studies have been performed in thisarea. Therefore, identification of microbial community and characterization of thepotential probiotic strains in biofloc-based aquaculture systems are needed. So, thisstudy used both culture-dependent and high-throughput 16S rRNA metagenomicsmethods to analyze the bacterial communities in probiotics, culture water, and the gutsof tilapia and shing fish reared in biofloc systems.