Single cell-type transcriptome profiling reveals genes that promote nitrogen fixation in the infected and uninfected cells of legume nodules

In this study, we have manually separated two main types of cells in mature nodules, known as infected cells (ICs) and uninfected cells (UCs). Smart-Seq2 mediated single-cell RNA sequencing reveals that 939 genes are significantly differentially expressed between ICs and UCs, including transcription factors, transporters, and catalytic enzymes. our data provide valuable resources for investigating genetic components responsible for the differentiated but coordinated cellular process between ICs and UCs. It also lays the foundation for improving the SNF efficiency in legume crops and for reconstructing the SNF system in non-legume crops. Overall design: Single-cell transcriptome profiling of the infected cells and uninfected cells in mature root nodules at 4 weeks post-inoculation.